MRET® ACTIVATED WATER AND ITS SUCCESFUL APPLICATION FOR PREVENTIVE TREATMENT AND ENHANCED TUMOR RESISTANCE IN ONCOLOGY
The goal of this investigation was to study on mice the effect of MRET® activated water as a potential agent for the prevention and treatment of two kinds of oncology diseases (laboratory models of Ehrlich's ascites tumor and Sarcoma ascites form). Significant positive effect of MRET® activated water regarding the tumor resistance on animals was observed in the experiments conducted on 500 mice (22 groups with 20 mice in each group and 10 groups with 5 mice in each group). The best results were observed in the groups of mice on MRET® water activated for 30 minutes (optimal regime of activation). The substantial anti-tumor efficacy was confirmed by very high level of reduction of Total Number of Viable Tumor Cells by 76% in “preventive treatment” and by 55% in “therapeutic treatment” regime. The life span of mice which received optimal activated water increased by 61% in “preventive treatment” and by 43% in “therapeutic treatment” regime. The significant anti-tumor effect of MRET® Activated Water on mice was close to the action of the chemotherapy agents and allowed to avoid the side effects that typically follow chemotherapy treatment in oncology.
The results of investigation of the application of water activated by non-ionizing Molecular Resonance Effect Technology (MRET®) process for prevention treatment and enhancement of tumor resistance of animals to two types of oncology diseases in vivo on 500 mice are presented. The research conducted on physical parameters of water confirmed that MRET® activation process contributed to substantial modification of the basic physical-molecular properties of distilled water (substantial reduction of viscosity as a function of applied tangent pressure, as well as substantial decrease of electrical conductivity and dielectric permittivity as functions of the frequencies of applied electromagnetic field). The significant positive effect of MRET® activated water on tumor resistance of animals was observed in the process of this investigation in all groups of mice on different fractions of activated water. The best results were observed in the groups of mice on MRET® water activated for 30 minutes (optimal regime of activation). The results were better in “preventive treatment” regime compare to “therapeutic treatment” regime. Additionally, this investigation confirmed that the long-term preservation of activated water at low temperature (around 0°C) for 45 days decreased its anti-tumor efficacy but left it on the significantly high level compare to other fractions. The test results point at the dual mechanism of MRET® water effect on tumors: the prevention and inhibition of tumor growth together with the reduction of quantity of viable tumor cells. The significant anti-tumor effect of MRET® activated distilled water on mice was close to the action of the chemotherapy agents and allowed to avoid the side effects that typically follow chemotherapy treatment of oncology.
In the process of investigation of cytotoxic activity of NK-cells the significant increase of lymphocyte cytotoxicity levels was observed when donor mice were treated with MRET® water activated for 30 minutes. The results also showed that the extension of the application of MRET® water from 14 days to 21 days significantly increased the value of cytotoxicity index. It is possible to admit that the extension of time of application of MRET® water will lead to higher levels of enhancement in NK-cells activity. Thus, the application of MRET® activated water can be quite promising approach for non-drug stimulation of NK-cells immunization vaccines.
The detailed description of this investigation is presented in BENEFITS MRET WATER.
The in vitro investigations on normal PMBC cells (peripheral blood mononuclear cell) and on HeLa cancer cells (cell line ATCC # CCL-2 cervical adenocarcinoma) and were conducted under the supervision of Patrick Pezzoli, Ph.D. at AltheaDx Technologies, San Diego, USA. The experiments analyzed: cells lysed at 0 hour, cells cultured for 24 hours in untreated medium and cells cultured for 24 hours in medium treated with MRET activator for 30 minutes. DNA samples from each batch were processed and the resultant data was analyzed using Affymetrix Genotyping Console 3.0 to obtain genotype calls and copy number calls. Cell counts and % viability were obtained using the Trypan Blue exclusion technique.
The experimental data revealed no difference between the zero hour (control), MRET treated and untreated samples in term of genotypes and copy number calls. Thus, MRET activation of water based medium did not induce any changes in cells on genetic level.
The studies showed that in MRET activated medium the viability of normal cells (PBMC) was higher (Table 1), and the viability of cancer cells (HeLa) was lower (Table 2) compared to their viability in untreated medium.
|Sample||Cell Count||% Viability||Viable cells|
|Sample||Cell Count||% Viability||Viable cells|
For normal cells (PBMC) the changes in cell counts were similar for untreated and MRET treated medium (Fig 1). Thus, MRET treatment did not affect the growth of normal cells.
For cancer cells (HeLa) the experimental data revealed significant inhibition of cancer cells growth in MRET treated medium. The growth of viable cancer cells was inhibited by 54% in MRET treated medium compared to untreated medium (Fig 2).
The results of AltheaDx research on HeLa cancer cells in vitro support the results obtained earlier in the investigation regarding the effects of MRET water on tumor resistance in animal model. The study on 500 mice was conducted under the supervision of Professor V. Vysotskii, S. Olishevsky, Ph.D. and Y. Yanish, Ph.D. at Kyiv Institute of Experimental Pathology, Oncology and Radiobiology of Ukrainian Academy of Science. It showed substantial inhibition of growth of viable tumor cells following the consumption of MRET water. In the course of this investigation the groups of mice in “preventive regime” ingested MRET water for 2 weeks prior to the inoculation of Ehrlich carcinoma cancer cells and for 3 weeks after the inoculation. The groups of mice in “therapeutic regime” ingested MRET water only during 3 weeks after the inoculation of Ehrlich carcinoma cancer cells. Following the consumption of MRET water activated for 30 minutes (the optimal time of activation) the growth of viable tumor cells was inhibited by 76% in “preventive regime” and by 55% in “therapeutic regime.”
As a conclusion it is possible to say that the studies conducted at AltheaDx Technology confirmed that MRET activated water did not affect the cells on genetic level; it affected the morphology of normal cells in positive way increasing their viability and promoted significant inhibition of growth of cancer cells.
A clinical observation was conducted on a patient undergoing chemotherapy treatment for metastasized naso-pharyngeal cancer. On a regular basis, WBC counts usually decrease to very low level. The rebounding of WBC from this low range takes about 3-5 weeks. The ingestion of MRET water prevented the decrease of WBC counts to their lowest level and helped to regain the pre-chemotherapy level within unusually short period of time of 3 days.
Thus, the clinical observation revealed that on the 10th day after the beginning of chemotherapy (in 3 days after WBC counts dropped to 10% of pre-chemotherapy level) WBC counts rebounded to 93% of their pre-chemotherapy level. In compliance with Student’s Distribution criteria the rebounding of WBC was higher than 70% of pre-chemotherapy level with p=0.05.
Testing conducted at C.A.I. Environmental Laboratory, Carlsbad, USA revealed the significant reduction of the amount of total coliforms following the process of MRET activation. In the rainwater activated for 30 minutes the amount of total coliforms decreased by 86% after the process of water activation. This test confirms that MRET water acts as antibacterial agent, providing sterilization effect.